PuroSPIN™ Endo-Free Plasmid Miniprep Kit #NKE111-50, #NKE111-250

PuroSPIN™ Endo-Free Plasmid Miniprep Kit is designed to provide simple and easy-to-use method for extraction and purification of plasmid DNA from cultured bacterial cells. The purification technology is based on plasmid binding to silica spin-columns, which allows efficient removal of genomic DNA, protein, salt and other contamits. Following the lysis steps, endotoxins are removed using the Endotoxin Removal Buffer ERB.

The PuroSPIN™ Endo-Free Plasmid Miniprep Kit can extract up to 15 µg of plasmid DNA with endotoxin levels <0.1 EU/µg. Silica spin columns offer faster, simpler and safer alternative to traditional nucleic acid purification techniques such as phenol-chloroform extraction. PuroSPIN™ buffer formulations produce highly purified plasmid DNA product for downstream applications, such as transfection, enzymatic digestion, or PCR.

Features:

• Purify endotoxin-free plasmid DNA from small volumes of bacterial culture
• Extract up to 15 µg of highly pure plasmid DNA with endotoxin levels <0.1 EU/µg

• Simply extraction process with no need for phenol or chloroform

• Optimized buffer formulations to ensure efficient removal of RNA from plasmid samples

• Efficient removal of genomic DNA, protein, salt, and other contamits

• High quality extracted plasmid DNA can be used for a range of downstream applications such as cloning, PCR, restriction enzyme digest, sequencing transformation, or transfection

SKU List:

NKE111-50, NKE111-250