PuroSPIN™ Endo-Free Plasmid MAXIprep Plus Kit #NKE213-10, #NKE213-30

PuroSPIN™ Endo-Free Plasmid MAXIprep Plus Kit is designed to provide simple and easy-to-use method for extraction and purification of plasmid DNA from cultured bacterial cells. The purification technology is based on plasmid binding to silica spin-columns, which allows efficient removal of genomic DNA, protein, salt and other contamits. Following the lysis steps, endotoxins are removed using the Endotoxin Removal Buffer ERB.

The PuroSPIN™ Endo-Free Plasmid MAXIprep Plus Kit can extraxt up to 500 µg of plasmid DNA with endotoxin levels of <0.1 EU/µg. Silica spin columns provide a faster, simpler, and safer alternative to traditional nucleic acid purification techniques such as phenol-chloroform extraction.

PuroSPIN™ buffer formulations produce highly purified plasmid DNA product for downstream applications, such as transfection, enzymatic digestion, or PCR.

Features:

• Purify endotoxin-free plasmid DNA from large volumes of bacterial culture
• Extraction of up to 500 µg of plasmid DNA while removing endotoxin to levels of <0.1 EU/µg
• Plus kit includes a syringe filter that quickly and easily removes insoluble fraction from the plasmid preparation, removing the need for the 12,000 x g centrifugation step
• Purify plasmid DNA from large volumes of bacterial culture
• Rapid purification of plasmid DNA (< 60 minutes), compared to ion-exchange column formats (> 3 hours)
• Efficient removal of genomic DNA, protein, salt, and other contamits
• High quality extracted plasmid DNA can be used for a range of downstream applications such as cloning, PCR, restriction enzyme digest, sequencing, transformation, or transfection

SKU List:

NKE213-10, NKE213-30