PuroMIX Direct Tissue PCR kit

PuroMIX Direct Tissue PCR Kit is specifically engineered for rapid and reliable amplification directly from animal tissues eliminating the need for conventional DNA extraction. Compatible sample types include fresh or frozen mammalian viscera, nails, hair, zebrafish, drosophila, and cultured cells.

The kit’s proprietary Direct Tissue Lysis Buffer A and B work synergistically to efficiently release genomic DNA from micro-tissues. This streamlined lysis process bypasses traditional purification steps, minimizing sample loss and significantly reducing experimental time and complexity.

PuroMIX 2X Direct Tissue PCR Mix is a ready-to-use master mix containing all essential PCR components, including:

Modified DNA polymerase with enhanced resistance to inhibitors and high-speed amplification capability.

Optimized enhancers that boost amplification efficiency and fidelity, enabling robust amplification of target sequences up to 3 kb.

Integrated loading dye for direct sample application to agarose gels, allowing fast visualization without compromising PCR performance.

Features: 

• No DNA extraction process
• Little sample required
• Save time 
• Minimize errors 

Storage

Direct Tissue Lysis Buffer A, store at 4 °C. 
Direct Tissue Lysis Buffer B and PuroMIX 2X Direct Tissue PCR Mix, store at -20 °C.
Valid for 12 months. Avoid repeated freezing and thawing