Protein G, Fluorescent Dye Labeled, 5 mg
Recombit Protein G that has been covalently labeled with a fluorescent dye.
The following fluorescence dye labels are available:
- AFDye 488
- AFDye 555
- AFDye 647
- IR750
- NOTE 1: AFDye 488 (similar to FITC), AFDye 555 (similar to Cy3), AFDye 647 (similar to Cy5) are analogs of AlexaFluor® 488, AlexaFluor® 555, and AlexaFluor® 647 dyes, respectively.
- NOTE 2: IR750 dye is ithe analog of the IRDye® 750 dye.
Recombit Streptococcus Protein G is produced in E. coli and is a non-glycosylated polypeptide chain containing 196 amino acids. Streptococcus Protein G is proprietarily produced in E. coli and purified via chromatographic techniques. Protein G binds through a non-immune mechanism specifically to the Fc region of IgG and other immunoglobuline subclasses.
The gene we selected to use for the production of Protein G originated from Streptococcus Strain G148, and was cloned and expressed in Escherichia coli. The regions of the gene corresponding to the albumin binding domains, the cell wall and cell membrane binding domains have been genetically deleted. however the Fc binding domain is still present. Our recombit Protein G contains 196 amino acid residues with predicted molecular mass of ~ 22.5 kDa, but migrates with an apparent molecular mass of ~ 30 kDa in SDS-PAGE.
The product is animal component free (ACF) and animal origin free (AOF)
Applications
Luna otech Protein G is provided for research laboratory or bioprocessing purposes only. Not for use in diagnostic or theraputic human or animal applications.
Formulation
Lyophilized powder
Storage
Store at 4°C if entire vial with lyophilized product will be used within 5 weeks. Store, frozen at -20°C . After reconstitution in distilled water or buffer, aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Specifications
| Size | AFDye 555, AFDye 647, AFDye 488, IR750 |